| Autor: |
Yu, J., Leung, W.K., Ebert, M.P.A., Ng, E.K.W., Go, M.Y.Y., Wang, H.B., Chung, S.C.S., Malfertheiner, P., Sung, J.J.Y. |
| Předmět: |
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| Zdroj: |
British Journal of Cancer; 7/1/2002, Vol. 87 Issue 1, p91, 7p |
| Abstrakt: |
Survivin was recently described as an apoptosis inhibitor. Its pathogenic role in gastric cancer is largely unknown. Expression of survivin in gastric cancer and non-cancer first-degree relatives, and its association with apoptosis and cyclo-oxygenase-2 expression was investigated. Fifty gastric cancer, 30 non-cancer first-degree relatives, 20 normal controls and five gastric cancer cell lines were studied. Survivin and cyclo-oxygenase-2 were evaluated by reverse transcriptase-polymerase chain reaction, immunohistochemistry and Western blot. Survivin expression was absent from normal gastric mucosa. All five cancer cell lines and 34 out of 50 (68%) human gastric cancer tissues expressed survivin mRNA. Survivin expression was less frequent (22%; P < 0.001) in adjacent non-tumour gastric tissues. Immunohistochemistry and Western blot obtained similar findings. Gastric cancers with survivin expression displayed significantly reduced apoptosis (P = 0.02), and associated with cyclo-oxygenase-2 overexpression at both mRNA (P = 0.001) and protein levels (P = 0.041). Moreover, survivin mRNA was detected in the gastric mucosa of eight (27%) non-cancer relatives. Expression in non-cancer patients showed positive correlation with H. pylori infection (P = 0.004). This demonstrates the frequent expression of survivin in gastric cancer and in first-degree relatives. Coexpression of survivin and cyclo-oxygenase-2 may suggest multiple pathways contributing to the inhibition of apoptosis in gastric cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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