| Autor: |
Pollastro, S., Angelini, R. M. De Miccolis, Gerin, D., Rotolo, C., Masiello, M., Miazzi, M., Santomauro, A., Ferrara, M., Pentimone, I., Fallanaj, F., Sanzani, S. M., Ippolito, A., Nigro, F., Faretra, F. |
| Předmět: |
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| Zdroj: |
Journal of Plant Pathology; Dec2012 Supplement, Vol. 94 Issue 4, pS4.75-S4.76, 2p |
| Abstrakt: |
In the framework of Project No. 14 "Laboratory network for the selection, characterization and conservation of germplasm and for preventing the spread of economically-relevant and quarantine pests (SELGE)", founded by the Apulia Region, PO FESR 2007-2013, an Illumina HiScan Seq platform was acquired. The next generation sequencing technologies (NGS) can be applied to improve knowledge in several contexts, such as genotyping, whole-genome sequencing, targeted resequencing, gene expression, discovery of transcription factor binding sites, noncoding RNA expression profiling, etc. With particular regard to transcriptomics, the application of NGS, commonly RNA-seq, allows the nearly complete characterization of transcriptomic events occurring in a specific biological system. Quantitative and qualitative differences in gene expression are detected by comparing multiple mRNA populations, and the understanding of fungal plant diseases can be improved. The analysis of gene expression during infection, together with expression profiling in a multitude of different in vivo and in vitro growth conditions can greatly enhance the understanding of fungal pathogenicity. The Illumina NGS was applied to gain insight into a wide range of transcriptional events related to: (i) plant-pathogen interaction (i.e grapevine-Phomopsis viticola); (ii) morphogenesis and development in Botryotina fuckeliana, with particular regard to mycelial growth, sclerotia differentiation, occurrence of the sexual process, and ascocarp development; (iii) synthesis of ochratoxin A in Aspergillus carbonarius grown under inducive and non-inducive condition; (iv) defoliating and non-defoliating pathotypes of Verticillium dahliae growing in a synthetic simulated xylem medium; (v) induced resistance to postharvest decay in oranges treated with electrolyzed water. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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