Non-suture technique for rabbit oviduct anastomosis with 2-octyl cyanoacrylate: A histopathologic and biomechanical analysis.

Autor: Zhu, Hua, Yang, Xiao‐Jun, Li, Xiao‐Lin, Zhang, Hu‐Xiang, Zheng, Fei‐Yun
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Zdroj: Journal of Obstetrics & Gynaecology Research; Mar2013, Vol. 39 Issue 3, p685-691, 7p, 1 Color Photograph, 1 Black and White Photograph, 1 Chart, 1 Graph
Abstrakt: Aim: The aim of this study was to investigate histological and biomechanical properties of oviduct anastomosis with 2-octyl cyanoacrylate (OCA) in the rabbit. Material and Methods: Sixty female rabbits were randomly divided equally into three groups: A (control), B (traditional catgut suture), and C (non-suture technique using OCA). After suture or OCA anastomosis, gross examination (adhesion formation) and histopathology (hematoxylin-eosin), ultrastructure (transmission electron microscopy), and biomechanics (bursting pressure) on para-anastomotic site were investigated on oviduct taken at 1 (A1, B1, C1) and 4 (A2, B2, C2) weeks, respectively. Results: Adhesion score in group B was more severe than that in groups A and C at 1 and 4 weeks. Histopathology showed that acute endosalpingitis in group B was the most intense at 1 week, followed by significantly more tissue stimulation induced by catgut and foreign-body giant cells in group B than in group C at 4 weeks. Ultrastructural damage of ciliated cells was reversed partly (B2) and completely (C2) at 4 weeks. Bursting pressure in C1 was weaker than that in B1, followed by no significant difference at 4 weeks. Conclusion: Non-suture using OCA for oviduct anastomosis can be accepted as a new-perspective technique. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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