First record and preliminary evaluation of Mucor hiemalis as biocontrol agent on inflorescence brown rot incidence of date palm.

Autor: Ziedan, El-Sayed H.E., Farrag, Eman S.H., Sahab, A.F.
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Zdroj: Archives of Phytopathology & Plant Protection; Mar2013, Vol. 46 Issue 5, p617-626, 10p
Abstrakt: In Egypt, inflorescence brown rot disease of date plam trees caused byThielaviopsis paradoxaDe Syenes causing high losses of pollen grains and fruits yield productivity. Infection occurs early on spathes even when it still hidden in the leaf bases. White mycelium of pathogenic fungi grows on inflorescence then turned to brown when fungus spores abundant. Isolation trails from diseased spathes showed brown rot, yielded three genus of fungali.e.Aspergillus niger(25%),Mucor hiemalis(25%) andT. paradoxa(50%). Pathogenicity test by using fungal isolates and male inflorescence of data indicate that, all isolates ofT. paradoxawere able to induce brown rot of inflorescence. Isolates ofT. paradoxawere differed in pathogenic activity for producing inflorescence brown rot symptom. Also,A. nigerisolate could cause slightly decay on inflorescence. Meanwhile, all isolatesof M. hiemalisrecorded as non pathogenic.In vitro, dual culture technique by usingM. hiemalisshowed antagonistic properties againstT. paradoxa. Scanning electron microscopy (SEM) study revealed that, pollen grains of date palm are susceptible to infection byT. paradoxa, accompanied by complete lyses and ruptured. SEM examination of inflorescence treated by each ofM. hiemalisor/andT. paradoxashowed thatM. hiemaliswas able to colonisation on inflorescence and reduced colonisation ofT. paradoxaon inflorescence and pollen grains. Preliminary evaluation ofM. hiemalisas a biocontrol agent showed that, spray of inflorescence withM. hiemalissuspension two days before or after infestation byT. paradoxawere reduced brown rot of inflorescence than the control. Spraying of spathes byM. hiemalisbefore infestation by pathogen was highly effective in reduction brown rot incidence compared with spraying after infestation. These results help to explain the role ofM. hiemalisin the suppression and biological control ofT. paradoxa. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index