| Autor: |
Nissim Ben Efraim, A. H., Karra, L., Ben‐Zimra, M., Levi‐Schaffer, F. |
| Předmět: |
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| Zdroj: |
Allergy; Mar2013, Vol. 68 Issue 3, p397-401, 5p, 2 Graphs |
| Abstrakt: |
Background Eosinophils are involved in several inflammatory processes including allergic inflammation. It has been shown that eosinophil functions may be regulated by activating or inhibitory receptors. Hypoxia is a feature of inflamed tissues and has recently been shown to regulate eosinophil viability and pro-angiogenic potential. In this study, the effect of hypoxia and GM- CSF on the inhibitory receptor CD300a in human peripheral blood eosinophils was investigated. Methods CD300a expression on eosinophils was analyzed by flow cytometry and evaluated by immuno-fluorescence; mRNA levels were evaluated by RT- PCR. Results An increase in the expression of CD300a was observed in hypoxic eosinophils compared to the normoxic ones. GM- CSF strongly induced CD300a increase also after 3 h in culture. In addition, hypoxia augmented mRNA levels of CD300a. Inhibition of hypoxia-inducible factor ( HIF)-1 abolished the hypoxia-/ GM- CSF-induced CD300a increase. Conclusion CD300a expression is up-regulated by hypoxia, and GM- CSF where HIF-1 might play an important role. These results are important for the understanding of eosinophils behavior in inflamed tissue and suggest a new effect on their function in allergic inflammation. Taken together our data point out CD300a as a novel target for the treatment of allergy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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