Substrate-like inhibition of the transgalactosylation reaction catalyzed by β-galactosidase from Aspergillus oryzae.

Autor:	Irazoqui, Gabriela, Bustamante, María José, Castilla, Agustín, Villagrán, Laura Virginia, Batista-Viera, Francisco, Brena, Beatriz M., Giacomini, Cecilia
Předmět:	CHEMICAL reactions GALACTOSIDASES KOJI ENZYME inhibitors GLYCOSIDASES CHEMICAL synthesis ENZYMES ETHYLENE glycol
Zdroj:	Biocatalysis & Biotransformation; Jan/Feb2013, Vol. 31 Issue 1, p57-65, 9p, 2 Charts, 4 Graphs
Abstrakt:	The influence of key parameters in the kinetics of the transglycosylation system catalyzed by Aspergillus oryzae β-galactosidase when applied to the synthesis of galactosyl-ethylene glycol and galactosyl-glycerol has been studied. It was found that both acceptors were inhibitors of the enzyme, causing a decrease of 9 and 68 fold in enzyme catalytic efficiency in the presence of 4.0 M ethylene glycol and glycerol, respectively. The effect was characterized as 'substrate-like inhibition', suggesting the formation of a nonproductive enzyme-acceptor complex. Inhibition constants for both acceptors were determined as 618 mM for ethylene glycol and 244 mM for glycerol. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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