| Autor: |
Kudo, Hideki, Nakayama, Tohru, Mano, Yoshihiro, Suzuki, Satoe, Sassa, Shuji, Sakamoto, Shinobu |
| Předmět: |
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| Zdroj: |
Cell Biology International; Feb2002, Vol. 26 Issue 2, p211, 5p |
| Abstrakt: |
Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G0/G1 arrest in mouse leukemia L1210 cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA-synthesizing enzymes thymidylate synthase and thymidine kinase within 6 h, and the cells entering into S phase within 12 h. The sequence was followed by the marked activation of both enzymes within 24 h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36 h. In conclusion, mouse leukemia L1210 cells, which were treated with 1.5% DMSO for 96 h, tolerated the treatment and reversed the cell cycle arrest within 36 h. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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