| Abstrakt: |
Myriophyllum sibiricum Komarov, an aquatic dicotyledonous macrophytewith a north temperate distribution, was assessed for use in a new bioassay to determine the effect of pesticides, agricultural runoff and municipal waste upon non-target aquatic macrophytes. An axenic culturing system was developed for which an optimal growth medium is required before a bioassay will be reliable. Five media (Murashige and Skoog, Hoagland's, Gaudet's, modified Andrew's, and Hard Water media), commonly used for aquatic plant culturing, were compared to determinethe effect on M. sibiricum growth and development. Morphological endpoints for the assay included shoot length, total root length and number, fresh weight and plant area. Membrane integrity, chlorophyll a, chlorophyll b and carotenoid content were the physiological endpointsexamined. Based upon these criteria, the modified Andrew's medium ata pH of 5.8, without the addition of a buffer was chosen as a mediumwhich supported rapid and consistent development of M. sibiricum during the two week assay period. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink