Studying the Influence of the Pyrene Intercalator TINA on the Stability of DNA i-Motifs.

Autor:	El-Sayed, AhmedA., Pedersen, ErikB., Khaireldin, NahidA.
Předmět:	PYRENE CYTOSINE NUCLEOTIDE sequence MOLECULAR structure OLIGONUCLEOTIDES PH effect POLYETHYLENE glycol
Zdroj:	Nucleosides, Nucleotides & Nucleic Acids; Dec2012, Vol. 31 Issue 12, p872-879, 8p
Abstrakt:	Certain cytosine-rich (C-rich) DNA sequences can fold into secondary structures as four-stranded i-motifs with hemiprotonated base pairs. Here we synthesized C-rich TINA-intercalating oligonucleotides by inserting a nonnucleotide pyrene moiety between two C-rich regions. The stability of their i-motif structures was studied by using UV melting temperature measurements and circular dichroism spectra at different pH values under noncrowding and crowding conditions (20% poly(ethylene glycol)). When TINA ((R)-3-((4-(1-pyrenylethynyl)benzyl)oxy) propane-1,2-diol) is inserted, the oligonucleotides could form an i-motif at a higher pH than observed for the corresponding wildtype oligonucleotide. [ABSTRACT FROM PUBLISHER]
Databáze:	Complementary Index
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