| Autor: |
Jia Yi Lin, Pitman-Crawford, Janet L., Bibby, Adrian H., Hudson, Norma L., Mcintosh, C. Joy, Juengel, Jennifer L., McNatty, Kenneth P. |
| Předmět: |
|
| Zdroj: |
Reproduction; Nov2012, Vol. 144 Issue 5, p557-567, 11p |
| Abstrakt: |
The aims were to investigate whether oocyte-secreted growth factors from a high (i.e. rat) and low (i.e. sheep) ovulation rate species could stimulate ³H-thymidine incorporation in granulosa cells (CC) from antral follicles from the same or across species. Denuded oocytes (DO) were co-incubated with CC with or without specific antibodies to growth differentiating factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15). Co-incubations of DO-CC from the same or across species significantly increased thymidine incorporation in CC with increasing numbers of DO. CDF9 immuno-neutralisation reduced thymidine incorporation in rat CC co-incubated with either rat or ovine DO and in ovine CC co-incubated with ovine or rat DO. BMP15 immuno-neutralisation only reduced thymidine incorporation when ovine DO were co-incubated with either ovine or rat GC. Western blotting of oocytes co-incubated with CC identified GDF9 and BMP15 proteins for sheep and GDF9 protein for rats in oocyte lysates and incubation media. With respect to rat BMP1 5, a promature protein was identified in the oocyte lysate but not in media. Expression levels of GDF9 relative to BMP15 mRNA in DO co-incubated with CC were highly correlated (R² = 0.99) within both species. However, the expression ratios were markedly different for the rat and sheep (4.3 vs1.O respectively). We conclude that during follicular development, rat oocytes secrete little, if any, BMP1 5 and that CDF9 without BMP15 can stimulate proliferation of rat and ovine CC. In contrast, ovine oocytes secrete both BMP15 and CDF9, and both were found to stimulate proliferation in ovine and rat CC. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
