| Autor: |
Hashizume, Rintaro, Smirnov, Ivan, Liu, Sharon, Phillips, Joanna, Hyer, Jeanette, McKnight, Tracy, Wendland, Michael, Prados, Michael, Banerjee, Anu, Nicolaides, Theodore, Mueller, Sabine, James, Charles, Gupta, Nalin |
| Zdroj: |
Journal of Neuro-Oncology; Dec2012, Vol. 110 Issue 3, p305-313, 9p |
| Abstrakt: |
Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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