Sodium bisulfite analyses in shrimp by ion selective electrode, sulfite oxidase, and modified Monier-Williams

Autor: Martin, R. M., Grodner, R. M., Gerdes, D. L., Hirchak, R. D.
Předmět:
Zdroj: Journal of Aquatic Food Product Technology; 1999, Vol. 8 Issue 1, p59, 0p
Abstrakt: Sulfite determination in shrimp was accomplished using an ion-selective electrode, and enzymatic method and the Modified Monier-Williams procedure. A rapid electrode method using a double junction-sleeve type electrode with a hydrogen reference electrolyte was developed using sodium metabisulfite (0.01 M) as the reference electrolyte and saturated silver chloride as the internal electrolyte. Concentrations between 0 and 250 ppm were tested to produce a standard curve. The concentration range of interest to the shrimp industry lies between 50 and250 ppm. Response time was approximately 90 s with slight variationsafter 120 s. This technique was completed in a fraction of the time required by the modified Monier-Williams and the enzyme method, whichtake 2.5 h and 45 min, respectively. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index