| Autor: |
Barkay, T., Devereux, R., Jonas, R. B., Kurtz, J. C. |
| Předmět: |
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| Zdroj: |
Environmental Toxicology & Chemistry; Jul1998, Vol. 17 Issue 7, p1274, 0p |
| Abstrakt: |
Microcosms consisting of estuarine sediment slurries were examined for their utility as models for assessing effects on microbial community structure and function. Data were obtained over a 2-week period toevaluate the reproducibility between individual microcosms and the variability between microcosm slurries and fresh sediment cores. Sulfate reduction rates in microcosm slurries did not differ significantlyfrom rates for freshly collected sediment cores (p >/= 0.05). However, the measured rates were more variable in microcosm slurries (SE = 10.03-0.25 nM/ml/h) than in freshly collected sediments (SE = 10.01-0.12 nM/ml/h). Rates of dark CO2, fixation in the microcosmslurries declined but were consistent with rates in freshly collected sediments (6.51 and 9.29 nM/ml/h on day 3, respectively). Relative abundances (RAs) of 16S rRNA determined for six specific phylogeneticassemblages of sulfate-reducing bacteria were reproducible among three microcosm replicates with Desulfovibrio spp. consistently in greatest abundance (RA = 8.611 1.40, day 7). Total direct bacterial countswere not significantly different between freshly collected sedimentsand microcosm slurries (p >/= 0.05). The results indicated that microcosms were both reproducible and representative of the field, and could thus provide a potentially useful tool for studies of microbial community response to perturbation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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