Characterisation of a cell-free supernatant obtained from cultures of Enterococcus faecalis DBFIQ E24 with antagonistic activity against bacteria, yeasts and moulds.

Autor: DE LAS M CARDOSO, MARÍA, MANZO, RICARDO M, TONARELLI, GEORGINA G, SIMONETTA, ARTURO C
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Zdroj: International Journal of Dairy Technology; Nov2012, Vol. 65 Issue 4, p568-577, 10p, 6 Charts, 3 Graphs
Abstrakt: Enterococcus faecalis DBFIQ E24 strain produces antimicrobial substances that inhibit the growth of food-borne pathogenic bacteria, yeasts and moulds. This broad antimicrobial spectrum was determined by obtaining the cell-free supernatant (CFS) and employing the agar-well-diffusion method. Its antibacterial activity was completely inactivated by the action of proteinase K and not affected by catalase action. Besides, it was stable to thermal treatments, showing maximum antibacterial activity at pH 7.0. Moreover, CFS showed a bactericidal effect against Escherichia coli DBFIQ Ec9 strain. All these interesting physicochemical properties allow that either CFS or E. faecalis DBFIQ E24 strain to be considered as potential food biopreservatives. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index