Resistin-like molecule-β is induced following bronchoconstriction of asthmatic airways.

Autor: GRAINGE, CHRISTOPHER, DULAY, VALDEEP, WARD, JONATHAN, SAMMUT, DAVID, DAVIES, ELIZABETH, GREEN, BENJAMIN, LAU, LAURIE, COTTEY, LAURA, HAITCHI, HANS-MICHAEL, DAVIES, DONNA E., HOWARTH, PETER H.
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Zdroj: Respirology; Oct2012, Vol. 17 Issue 7, p1094-1100, 7p
Abstrakt: ABSTRACT Background and objective: Resistin-like molecule-β (RELM-β) is a necessary and sufficient stimulus for airway remodelling in animal models of asthma, but until recently, its role in human disease had not been investigated. The hypothesis that RELM-β expression would increase with increasing asthma severity and further increase following acute bronchoconstrictor challenges has been examined. Methods: Bronchial biopsies from healthy subjects and patients with mild and severe asthma were immunostained for RELM-β, as were airway biopsies obtained in mild asthmatics before and 4 days after repeated inhalation challenges with either allergen, methacholine or methacholine preceded by salbutamol as a control. Bronchial brushings were also evaluated for RELM-β mRNA. Results: RELM-β immunoreactivity, which co-localized to airway epithelial cells, increased with disease severity; healthy volunteers, median per cent epithelial area 1.98%, mild asthma 3.49% and severe asthma 5.89% ( P < 0.001 between groups). RELM-β immunoreactivity significantly and inversely correlated in asthma with forced expiratory volume in 1 s % predicted ( P = 0.005). Acute changes in immunoexpression were evident after repeated inhalation challenge with allergen (2.15 % to 4.35 % ( P = 0.01)) and methacholine (4.21 % to 6.16 % ( P = 0.01)) but did not change in the salbutamol/methacholine challenge group. These changes correlated with change in basement membrane thickness (r = 0.38, P = 0.02). Epithelial RELM-β gene expression was not altered in asthma. Conclusions: RELM-β may play an important role not only in animal models of airway remodelling, but also in human airway pathology. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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