| Autor: |
RIBEIRO, LAIR G. T., HILLIS, L. DAVID, LOUIE, ERIC K., DAVIS, MICHAEL A., MAROKO, PETER R., BRAUNWALD, EUGENE |
| Zdroj: |
Cardiovascular Research; Jun1978, Vol. 12 Issue 6, p334-340, 7p |
| Abstrakt: |
In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min−1·100 g−1. In myocardium with flow reduced to 0 to 15 cm3·min−1·100 g−1, creatine kinase activity was 7.6 ± 0.6 IU·mg−1 protein in control dogs and 13.1 ± 1.8 IU·mg−1 protein (P<0.01) in dogs given 500 NF units·kg−1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min−1·100 g−1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg−1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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