| Autor: |
Miyagawa, Shuji, Yamada, Mako, Matsunami, Katsuyoshi, Koresawa, Yukie, Ikawa, Masahito, Okabe, Masaru, Shirakura, Ryota |
| Předmět: |
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| Zdroj: |
Journal of Biochemistry; 2001, Vol. 129 Issue 5, p795-801, 7p |
| Abstrakt: |
The human DAF (CD55) gene was chosen as a representative molecule in a xenotrans-plantation study. The gene was synthesized in order to adapt its codons to those which are more frequent in mammals, especially pigs, and the expression levels were then examined in Chinese hamster ovarian (CHO) cells, swine endothelial cell (SEC) and transgenic mice. A significant increase in protein production with no detectable mRNA elevation was observed in the transfectants of synthetic DAF (sDAF), compared with the wild-type DAF (wtDAF) and delta-SCRl wild-type DAF (Δ1wtDAF). Consistent with the in vitro data, the expression of DAF in mice that carry sDAF was higher than AlwtDAF in many organs, especially the pancreas. The sDAF showed a high level of expression in SEC and transgenic mice, suggesting that it will be useful in the development of transgenic pigs with high levels of expression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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