| Abstrakt: |
The study aimed to study the influence of different reaction temperatures on the carp ( Cyprinus carpio) hepatic CYPs activity. Six groups of carp hepatic microsomes were incubated with probe drug (chorzoxazone) at 5, 10, 15, 20, 25 and 30°C separately. According to the principle of enzyme kinetics theory, the Michaelis constant ( K) value and maximum reaction velocity ( V) of CYPs (with CZX as probe) were obtained. The CYPs activity at different reaction temperatures was compared. In results, the K values were separately 44.62, 31.35, 26.59, 21.75, 16.39, 29.69 μM, and the V were separately 0.231, 0.234, 0.265, 0.294, 0.315, 0.239 nmol minmg at 5, 10, 15, 20, 25, 30°C. Results indicated that the enzyme CYPs activity was much higher at 25°C. It was also demonstrated that reaction temperature could affect the CYPs activity significantly. Therefore, this experiment builded a theoretical basis for the variations of fish pharmacokinetic parameters at different water temperatures and contributed to further research on the influence of water temperature on fish drug metabolism. [ABSTRACT FROM AUTHOR] |
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