| Autor: |
Lewis, D. A., Kanhere, S. R., Lau, B. P.-Y., Scott, P. M., Ryan, J. J., Hayward, S., Kuiper-Goodman, T. |
| Předmět: |
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| Zdroj: |
Food Additives & Contaminants; Jul1998, Vol. 15 Issue 5, p555, 0p |
| Abstrakt: |
Blood plasma samples collected from 1144 healthy volunteers in 16 locations across Canada in 1994 were analysed for ochratoxin A (OTA). The method of analysis included cleanup by C18 solid phase extraction and immunoaffinity columns followed by, liquid chromatography (LC) with fluorescence detection, which gave 86.5% (s.d. = 9.7) recovery (n = 31) of OTA (added at 2 ng/ml) with a detection limit 0.15 ng/m. The arithmetic mean concentration found in the plasma samples, corrected for volume of anticoagulant added, was 0.88 ng/ml with a standard deviation 0.35 ng/ml and a range of 0.29- -2.37ng/ml. Confirmation of identity of OTA was by methyl ester formation for samples and by LC-tandem mass spectrometry for 17 samples (some of which were included in pooled samples). Statistical analysis, by ANOVA of the log OTA plasma concentrations, showed a highly significant effect due to location in Canada (p=<0.0001) but no effect due to age, sex or blood group of donors. The highest mean concentration was found in Winnipeg, significantly different (p = 0.05) by the Student-Newman-Keuls multiple range test from the lowest levels found in Toronto, Vancouverand Saint John. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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