| Autor: |
Bamberger, David M., Gerding, Dale N., Bettin, Kris M., Elson, Michael K., Forstrom, Lee A. |
| Zdroj: |
Journal of Infectious Diseases; 1985, Vol. 152 Issue 5, p903-912, 10p |
| Abstrakt: |
Rabbit peripheral blood and glycogen-stimulated peritoneal neutrophils were labeled with [111In]indium oxine and transfused intravenously into recipient rabbits with experimental abdominal abscesses due to Staphylococcus aureus. Peritoneal neutrophils harvested 4 hr after glycogen infusion localized within the abscesses to a greater extent than did peripheral blood neutrophils (P < .002). In an in vitro chemotaxis under-agarose assay, peripheral blood neutrophils had greater random migration (P < .002) and directed migration (P < .01) than did peritoneal cells. In an in vitro glass slide adherence assay, peritoneal neutrophils were more adherent than were blood neutrophils (P < .05). The discrepancy between in vivo and in vitro findings may be due to the increased adherence of peritoneal neutrophils. Glycogen-stimulated peritoneal neutrophils have been exposed in vivo to C5a, which is known to decrease migration and increase adherence in vitro of polymorphonuclear neutrophils; consequently, in vivo exposure of neutrophils to C5a may mean in vitro migration data may be misleading in predicting results in vivo. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
