Autor: |
Park, Seung-Hyun, Han, Kwang-Seok, Kim, Tae-Wuk, Shim, Jae-Kuk, Takatsuto, Suguru, Yokota, Takao, Kim, Seong-Ki |
Předmět: |
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Zdroj: |
Plant & Cell Physiology; Sep1999, Vol. 40 Issue 9, p955-960, 6p |
Abstrakt: |
Identification by full-scan GC-MS revealed that [2H6]-teasterone fed to suspension cultured cells of Marchantia polymorpha was converted to [2H6]3-dehydroteasterone and [2H6]typhasterol. This indicates that the cells carry out a C3-epimerization in which teasterone is converted to typhasterol via 3-dehydroteasterone. In vitro enzymatic conversions of teasterone to typhasterol were also investigated. A crude cytosolic solution prepared from Marchantia cells catalyzed not only the dehydrogenation of teasterone to 3-dehydroteasterone but also the reduction of 3-dehydroteasterone to typhasterol. The major 4-demethysterol in cultured M. polymorpha cells was 24-methylcholesterol, the precursor of brassinosteroids. These results suggest that enzymes similar to those involved in the early C-6 oxidation pathway of the brassinosteroid biosynthesis are present in the liverwort. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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