| Autor: |
Tiuriaeva, I., Kuranova, M., Gonchar, I., Rozanov, Yu. |
| Zdroj: |
Cell & Tissue Biology; Jul2012, Vol. 6 Issue 4, p367-375, 9p |
| Abstrakt: |
The effect of the metabolic drug Cytoflavin (CF) with antihypoxic and antioxidative properties on human dermal fibroblasts in the model of ischemia/reoxygenation in vitro was studied. It was revealed that, during the reoxygenation period after ischemia, the cell death rate in culture with CF appeared to be 2-2.7 times lower than in control cultures. Recovery of ATP synthesis after ischemia was 2.1 times faster in culture with CF. The drug effectively reduced the level of reactive oxygen species (ROS) in fibroblasts after HO treatment, which allowed their survival to be maintained at the level of control cells. Pretreatment of cells with CF for one day ensured the maintenance of a normal level of ROS during the investigated time period in fibroblasts subjected to HO treatment and reduced HO-induced cell death by almost one-third as compared to non-pretreatment control cells. Addition of CF into the culture medium in the postischemic period showed no effect on Hsp70 synthesis, but led to a decrease in GRP78 synthesis, raised after ischemia, to the control level, indicating a resolution of unfolded protein response in these cells and normalization of the functioning of their endoplasmic reticulum. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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