| Autor: |
Graham, John S., Bryant, Mark A., Brave, Ernest H. |
| Zdroj: |
Journal of Toxicology -- Cutaneous & Ocular Toxicology; 1994, Vol. 13 Issue 1, p47-54, 8p |
| Abstrakt: |
The skin of 24 anesthetized hairless guinea pigs was exposed to saturated sulfur mustard (bis-2-chloroethyl sulfide; HD) vapor for 5 and 7 min (24 sites per exposure) using 14 mm diameter vapor cups. The animals were euthanized 24 hr after HD exposure and skin specimens were taken and fixed for morphometric evaluation for granulated mast cells with an image analysis system (IAS). A total of 32 exposure sites was taken and evaluated from four naive guinea pigs. All tissue specimens were processed in paraffin and sectioned at 5 μm. One section each was stained with Unna's stain for mast cells and hematoxylin and eosin (H&E) and evaluated by light microscopy. An average area of 6.48 × 105 μm2 of skin was examined per site with the IAS. The Unna's stain together with interference light filters on the IAS enhanced the contrast between mast cell granules and the background tissue, allowing the number of granulated mast cells to be determined. The number of pixels detected by the IAS represented the area occupied by mast cell granules. There were significantly fewer mast cells (p < 0.05) in either the 5 or 7 min HD exposure groups than in the sham-exposed animals, with significantly fewer mast cells in the 7 min HD than the 5 min HD group. There were also significantly smaller areas occupied by granules in either HD-exposed group than in sham-exposed animals. HD-induced lesions in the hairless guinea pig model have shown signs of an inflammatory response, and with their granules of vasoactive histamine, mast cells might be expected to play a role in HD-induced injury. Changes in mast cells exposed to low sulfur mustard levels, as detected by an IAS, may serve as an early marker for cutaneous damage, which might not be as easily determined with routine light microscopic examination. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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