Regulatory T-cell subset analysis and profile of interleukin (IL)-10, IL-17 and interferon-gamma cytokine-producing cells in kidney allograft recipients with donor cells infusion.

Autor: Ranjbar, Moslem, Solgi, Ghasem, Mohammadnia, Mousa, Nikbin, Behrouz, Pourmand, Gholamreza, Ansaripour, Bita, Amirzargar, Aliakbar
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Zdroj: Clinical & Experimental Nephrology; Aug2012, Vol. 16 Issue 4, p636-646, 11p
Abstrakt: Background: This pilot study aimed to assess whether the perioperative infusion of donor bone marrow cells (DBMC) in renal allograft recipients can affect the appearance of peripheral regulatory T-cell subsets and the profile of cytokine-producing cells [interferon-gamma (IFN-γ), interleukin (IL)-17 and IL-10] 2 years after transplantation. Methods: Fresh blood samples were collected from 14 kidney recipients who received infusion and from 13 kidney recipients without infusion who served as controls at the end of the second post-transplantation year. Initially the percentages of CD4CD25FoxP3 T cells and CD3CD8CD28 T cells were quantified using flowcytometry. Thereafter, the frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined separately using the ELISPOT technique with peptides corresponding to mismatched donor HLA-DR molecules and phytohemagglutinin (PHA). Results: The mean numbers of IFN-γ- and IL-17-producing cells in response to PHA were lower in infused patients than in controls ( P = 0.02 and P = 0.18, respectively); however, an increased frequency of IL-10-producing cells was observed compared to controls ( P = 0.07). Furthermore, the ratio of IL-10/IFN-γ-producing cells was significantly higher in the DBMC-infused group versus controls ( P = 0.01). There was a negative correlation between the percentage of CD3CD8CD28T cells and IL-17-producing cells in the infused group ( r = −0.539, P = 0.04). The mean levels and the frequency of microchimerism within the first post-transplantation year were also significantly higher in infused patients than in controls ( P = 0.007 and P = 0.001, respectively). Conclusion: Our findings suggest that DBMC infusion could partially stimulate the regulatory mechanisms against alloimmune responses in kidney allograft recipients. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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