| Autor: |
Ho, Begonia Y., Current, Lori, Drewett, James G. |
| Předmět: |
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| Zdroj: |
FEBS Letters; Jul2002, Vol. 522 Issue 1-3, p130, 5p |
| Abstrakt: |
The cannabinoid CB1 but not the CB2 receptor was demonstrated to couple via Gα16 to activate phospholipase C after co-expression in COS7 cells. Chimeric CB1/CB2 receptors were used as a model to study receptor–Gα16 interaction. Sequences of the second and third intracellular loops and the carboxy-terminus were substituted from the CB1 into the CB2 receptor. Only the triple mutant with all three regions replaced activated phospholipase C to a similar extent as the CB1 receptor, suggesting that all three intracellular regions are required for interacting with Gα16. Several sub-domains within the third intracellular loop were identified for receptor–Gα16 interaction. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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