Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods.

Autor: Eshraghi, S., Salehipour, Z., Pourmand, M. R., Forushani, A. Rahimi, Salehi, M. T. Zahraei, Amiri, S. Agha, Bakhtyari, R., Mohtasab, T. P. Abedi, Mardani, N., Amiri, S. Seyed, Dallal, M. M. Soltan
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Zdroj: Tehran University Medical Journal; Oct2009, Vol. 67 Issue 7, p470-476, 7p
Abstrakt: Background: Staphylococcus aureus is a major foodborne pathogen throughout the world. Enterotoxins and toxic shock syndrome toxin-1 are important virulence factors and as pyrogenic toxin superantigens have profound effects on the ir host. Thus circulation of TSST1 producing S.aureus among people and food chain is a worrying issue. The present paper was conducted to study Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods. Methods: Over 1040 food samples have been analyzed differentially according to Iran national standard (number= 1194) for S.aureus identification. After DNA extraction, PCR reactions were carried out by reference strain as positive control, adequate primers. Results: At present study, prevalence of foodstuffs contaminated by S.aureus isolates was about 9.5% (100 strains). Of 25% of isolates producing entC, 28% (seven strains) had tst gene at the same time and of 8% of isolates producing entA, 12.5% (one strain) were positive for tst genes simultaneously. Altogether of 9% isolates producing combination of entC and entA, 44.4% (four strains) were also producer of tst gene. Conclusion: Prevalence of TSST1 producing strains in combination with enterotoxin genes is considerable especially with entC and A plus C. On the other hand, circulation of these isolates in humans, animals, foods and environment has hazardous effect for general public health. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index