Complete genome and pathogenicity of Tomato yellow leaf distortion virus, a bipartite begomovirus infecting tomato in Cuba.

Autor: Fiallo-Olivé, E., Hernández-Zepeda, C., Trejo-Saavedra, D., Carrillo-Tripp, J., Rivera-Bustamante, R., Martínez-Zubiaur, Y.
Zdroj: European Journal of Plant Pathology; Sep2012, Vol. 134 Issue 1, p13-21, 9p
Abstrakt: Tomato plants showing symptoms of yellow mottle and leaf distortion, first observed in eastern Cuba in 2007, have been shown to be associated with the presence of a novel bipartite begomovirus (genus Begomovirus, family Geminiviridae) species, Tomato yellow leaf distortion virus (ToYLDV), for which the DNA-A has already been sequenced. Here, we present the completion of ToYLDV genome characterization by cloning and sequencing the DNA-B component. In addition, we constructed infectious clones that were used to inoculate tomato, soybean, Nicotiana tabacum and N. benthamiana plants by a biolistic procedure. Cloned ToYLDV reproduced the symptoms observed in tomato in the field, thus confirming that this begomovirus is the causal agent of the disease present in Cuba. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index