| Autor: |
Shintani, David K., Cheng, Zigang, DellaPenna, Dean |
| Předmět: |
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| Zdroj: |
FEBS Letters; Jan2002, Vol. 511 Issue 1-3, p1, 5p |
| Abstrakt: |
A putative 2-methyl-6-phytylbenzoquinone (MPBQ) methyltransferase gene, SLL0418, was identified from the Synechocystis PCC6803 genome based on its homology to previously characterized γ-tocopherol methyltransferases. Genetic and biochemical evidence confirmed open reading frame (ORF) SLL0418 encodes a MPBQ methyltransferase. An SLL0418 partial knockout mutant accumulated β-tocopherol with no effect in the overall tocopherol content of the cell. In vitro assays of the SLL0418 gene expressed in Escherichia coli showed the enzyme efficiently catalyzes methylation of ring carbon 3 of MPBQ. In addition, the enzyme also catalyzes the methylation of ring carbon 3 of 2-methyl-6-solanylbenzoquinol in the terminal step of plastoquinone biosynthesis. [Copyright &y& Elsevier] |
| Databáze: |
Complementary Index |
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Plný text