| Autor: |
Zoval, Jim, Jia, Guangyao, Kido, Horacio, Kim, Jitae, Kim, Nahui, Madou, Marc |
| Zdroj: |
Springer Handbook of Nanotechnology (9783540298557); 2007, p549-570, 22p |
| Abstrakt: |
In this chapter centrifuge-based microfluidic platforms are reviewed and compared with other popular microfluidic propulsion methods. The underlying physical principles of centrifugal pumping in microfluidic systems are presented and the various centrifuge fluidic functions such as valving, decanting, calibration, mixing, metering, heating, sample splitting, and separation are introduced. Those fluidic functions have been combined with analytical measurements techniques such as optical imaging, absorbance and fluorescence spectroscopy and mass spectrometry to make the centrifugal platform a powerful solution for medical and clinical diagnostics and high-throughput screening (HTS) in drug discovery. Applications of a compact disc (CD)-based centrifuge platform analyzed in this review include: two-point calibration of an optode-based ion sensor, an automated immunoassay platform, multiple parallel screening assays and cellular-based assays. The use of modified commercial CD drives for high-resolution optical imaging is discussed as well. From a broader perspective, we compare the technical barriers involved in applying microfluidics for sensing and diagnostic as opposed to applying such techniques to HTS. The latter poses less challenges and explains why HTS products based on a CD fluidic platform are already commercially available, while we might have to wait longer to see commercial CD-based diagnostics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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