| Autor: |
Martín-Robles, Águeda, Whitmore, David, Sánchez-Vázquez, Francisco, Pendón, Carlos, Muñoz-Cueto, José |
| Předmět: |
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| Zdroj: |
Journal of Comparative Physiology B: Biochemical, Systemic & Environmental Physiology; Jul2012, Vol. 182 Issue 5, p673-685, 13p, 1 Diagram, 2 Charts, 6 Graphs |
| Abstrakt: |
An extensive network of endogenous oscillators governs vertebrate circadian rhythmicity. At the molecular level, they are composed of a set of clock genes that participate in transcriptional-translational feedback loops to control their own expression and that of downstream output genes. These clocks are synchronized with the environment, although entrainment by external periodic cues remains little explored in fish. In this work, partial cDNA sequences of clock genes representing both positive ( Clock) and negative ( Period1, Period2) elements of the molecular feedback loops were obtained from the nocturnal flatfish Senegalese sole, a relevant species for aquaculture and chronobiology. All of the above genes exhibited high identities with their respective teleost clock genes, and Per-Arnt-Sim or basic helix-loop-helix binding domains were recognized in their primary structure. They showed a widespread distribution through the animal body and some of them displayed daily mRNA rhythms in central (retina, optic tectum, diencephalon, and cerebellum) and peripheral (liver) tissues. These rhythms were most robust in retina and liver, exhibiting marked Period1 and Clock daily oscillations in transcript levels as revealed by ANOVA and cosinor analysis. Interestingly, expression profiles were inverted in retina and optic tectum compared to liver. Such differences suggest the existence of tissue-dependent zeitgebers for clock gene expression in this species (i.e., light for retina and optic tectum and feeding time for liver). This study provides novel insight into the location of the molecular clocks (central vs . peripheral) and their different phasing and synchronization pathways, which contributes to better understand the teleost circadian systems and its plasticity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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