| Autor: |
Morris, Gwilym M., Boyett, Mark R., Yanni, Joseph, Billeter, Rudolf, Dobrzynski, Halina |
| Zdroj: |
Cardiac Electrophysiology Methods & Models; 2010, p187-212, 26p |
| Abstrakt: |
Since the 1950s, technological advances have forged molecular biology into one of the most powerful fields of science. The primary molecular specializations of the cardiac conduction system are a lower expression of the fast Na+ channel (Nav1.5), the background K+ channel (Kir2.1), and the high conductance connexin (Cx43), but with a higher expression of the pacemaker channel (HCN4) and the alternative L-type Ca2+ channel (Cav1.3). Therefore, it is possible to investigate gene transcription and protein expression for these channels. This chapter describes the use of in situ hybridization, qPCR, and immunohistochemistry to study cardiac ion channel expression. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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