| Autor: |
Kamal, Manal, Abu Gabal, Khadiga, El-Naggar, Samia, Shaaban, Yasser Hassan, Shehata, Mohamed |
| Předmět: |
|
| Zdroj: |
Anatolian Journal of Obstetrics & Gynecology; 2011, Issue 1, p1-5, 5p |
| Abstrakt: |
Objective: Breast cancer causes major part of cancer deaths in women and is increasing in incidence. The tumor suppressor gene, BRCA1 has been conferred to increase the susceptibility to breast cancer. We aimed to determine the significance of BRCA1 gene expression in relation to other prognostic factors in Egyptian women. Materials and methods: Thirty patients with positive family history of breast cancer were selected from Beni-Suef University and Cairo University Hospitals, Egypt for the study. A control group of 10 healthy subjects were also included. RNA extraction from breast cancer tissues was done followed by real-time reverse transcription polymerase chain reaction (RT-PCR). BRCA1 expression was assessed and correlated with age, histological type and grade of breast cancer, estrogen and progesterone receptor status, and CA15-3 levels. Results: BRCA1 mRNA was found in 6 patients' samples (20% of the breast cancer tissues) while remaining patients (80%) were negative as well as the control group. A positive significant relationship was demonstrated between BRCA1 (mRNA) expression and high histological grade, negative estrogen and progesterone receptor status, and high levels of serum CA15-3. A significant negative correlation was found between BRCA1 mRNA expression and age. Conclusion: The study demonstrated the lack of BRCA1 gene expression (mRNA) in the majority of breast cancer cases and confirmed the relationship between BRCA1 expression and parameters that determine the poor prognosis in breast cancer in Egyptian women. Our results confirmed the role of BRCA1 in our study population. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
