| Abstrakt: |
A green, embryogenic callus line of lavender was obtained, analyzed, and compared with a long term, cultivated friable, brown callus from a morphological, biological, and biochemical view. Different morphogenetic responses, growth variables, enzymatic activities and isoenzyme patterns were registered for each line. A clear influence of growth regulator balance and combination was observed with benzyladenine and napthalene acetic acid being more efficient than kinetin and 2,4-dichlorophenoxyacetic acid in promoting callus growth and morphogenesis. Chloroplast formation and growth acceleration were associated with the appearance of compact, proembryogenic masses, while embryo development was correlated with lower growth indices, increased protein content, and higher levels of enzymatic activity. Isoperoxidase and isoesterase patterns indicated a certain polymorphism from one sample to another that was related to cell specialization during tissue differentiation. [ABSTRACT FROM PUBLISHER] |
