| Autor: |
Nakamori, Yoshiki, Liu, Bing, Ohishi, Kohshi, Suzuki, Kei, Ino, Kazuko, Matsumoto, Takeshi, Masuya, Masahiro, Nishikawa, Hiroyoshi, Shiku, Hiroshi, Hamada, Hirofumi, Katayama, Naoyuki |
| Předmět: |
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| Zdroj: |
British Journal of Haematology; Jun2012, Vol. 157 Issue 6, p674-686, 13p, 1 Chart, 6 Graphs |
| Abstrakt: |
The regulation of human early lymphopoiesis remains unclear. B- and T-lineage cells cannot develop simultaneously with conventional stromal cultures. Here we show that telomerized human bone marrow stromal cells supported simultaneous generation of CD19+ CD34lo/− CD10+cy CD79a+ CD20+/−Vpre B− pro- B cells and CD7+ CD34+ CD45 RA+ CD56−cy CD3− early T/Natural Killer ( NK) cell precursors from human haematopoietic progenitors, and the generation of both lymphoid precursors was promoted by flt3 ligand (flt3 L). On the other hand, stem cell factor or thrombopoietin had little or no effect when used alone. However, both acted synergistically with flt3 L to augment the generation of both lymphoid precursors. Characteristics of these lymphoid precursors were evaluated by gene expression profiles, rearrangements of Ig H genes, or replating assays. Similar findings were observed with primary human bone marrow stromal cells. Notably, these two lymphoid-lineage precursors were generated without direct contact with stromal cells, indicating that early B and T/ NK development can occur, at least in part, by stromal cell-derived humoral factors. In serum-free cultures, flt3 L elicited similar effects and appeared particularly important for B cell development. The findings of this study identified the potential of human bone marrow stromal cells to support human early B and T lymphopoiesis and a principal role for flt3 L during early lymphopoiesis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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