| Autor: |
Werner, Anniek, Minich, Deanna M., Havinga, Rick, Bloks, Vincent, Van Goor, Harry, Kuipers, Folkert, Verkade, Henkjan J. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Gastrointestinal & Liver Physiology; Oct2002, Vol. 283 Issue 4, pG900, 9p, 8 Charts, 8 Graphs |
| Abstrakt: |
Essential fatty acid (EFA) deficiency induces fat malabsorption, but the pathophysiological mechanism is unknown. Bile salts (BS) and EFA-rich biliary phospholipids affect dietary fat solubilization and chylomicron formation, respectively. We investigated whether altered biliary BS and/or phospholipid secretion mediate EFA deficiency-induced fat malabsorption in mice. Free virus breed (FVB) mice received EFA-containing (EFA[sup +]) or EFAdeficient (EFA[sup -]) chow for 8 wk. Subsequently, fat absorption, bile flow, and bile composition were determined. Identical dietary experiments were performed in multidrug resistance gene-2-deficient [Mdr2[sup (-/-)]] mice, secreting phospholipidfree bile. After 8 wk, EFA -fed wild-type [Mdr2[sup +/+]] and Mdr2[sup (-/-)] mice were markedly EFA deficient [plasma triene (20:3n-9)-to-tetraene (20:4n-6) ratio >0.2]. Fat absorption decreased (70.1 ± 4.2 vs. 99.1 ± 0.3%, P < 0.001), but bile flow and biliary BS secretion increased in EFA[sup -] mice compared with EFA[sup +] controls (4.87 ± 0.36 vs. 2.87 ± 0.29 µl·min[sup -1] · 100 g body wt[sup -1], P < 0.001, and 252 ± 30 vs. 145 ± 20 nmol·min[sup -1] · 100 g body wt[sup -1], P < 0.001, respectively). BS composition was similar in EFA[sup +]- and EFA[sup -]-fed mice. Similar to EFA[sup -] Mdr2[sup (+/+)] mice, EFA[sup -] Mdr2[sup (-/-)] mice developed fat malabsorption associated with twofold increase in bile flow and BS secretion. Fat malabsorption in EFA-mice is not due to impaired biliary BS or phospholipid secretion. We hypothesize that EFA deficiency affects intracellular processing of dietary fat by enterocytes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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