| Autor: |
Bagby, Susan P., LeBard, Linda S., Zaiming Luo, Ogden, Bryan E., Corless, Christopher, McPherson, Elizabeht D., Speth, Robert C. |
| Předmět: |
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| Zdroj: |
American Journal of Physiology: Renal Physiology; Oct2002, Vol. 52 Issue 4, pF755, 10p, 4 Color Photographs, 6 Black and White Photographs, 2 Charts, 10 Graphs |
| Abstrakt: |
To identify an appropriate model of human renin-angiotensin system (RAS) involvement in fetal origins of adult disease, we quantitated renal ANG II AT[sub 1] and AT[sub 2] receptors (ATIR and AT2R, respectively) in fetal (90-day gestation, n = 14), neonatal (3-wk, n = 5), and adult (6-mo, n = 8) microswine by autoradiography ([sup 125]I-labeled [Sar¹Ile[sup 8]]ANG II+cold CGP-42112 for AT1R, [sup 125]I-CGP-42112 for AT2R) and by whole kidney radioligand binding. The developmental pattern of renal ATIR in microswine, like many species, exhibited a 10-fold increase postnatally (P < 0.001), with maximal postnatal density in glomeruli and lower density AT1R in extraglomerular cortical and outer medullary sites. With aging, postnatal ATIR glomerular profiles increased in size (P < 0.001) and fractional area occupied (P < 0.04), with no change in the number per unit area. Cortical levels of AT2R by autoradiography fell with age from ≅5,000 fmol/g in fetal kidneys to ≅60 and 20% of fetal levels in neonatal and adult cortex, respectively (P < 0.0001). The pattern of AT2R binding in postnatal pig kidney mimicked that described in human and simian, but not rodent, species: dense AT2R confined to discrete cortical structures, including pre- and juxtaglomerular, but not intraglomerular, vasculature. Our results provide a quantitative assessment of ANG II receptors in developing pig kidney and document the concordance of pigs and primates in developmental regulation of renal ATIR and AT2R. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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