QUANTITATION OF A NEW MACROLIDE IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH TANDEM MASS SPECTROMETRIC DETECTION: COMPARISON WITH ELECTROCHEMICAL DETECTION.

Autor: Daszkowski, D.J., Ong, V.S., Menacherry, S., El-Shourbagy, T., Carroll, S.L., Marsh, K.C.
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Zdroj: Journal of Liquid Chromatography & Related Technologies; 3/1/99, Vol. 22 Issue 5, p641-657, 17p, 1 Chart
Abstrakt: A method involving high performance liquid chromatography with tandem mass spectrometric detection was developed to determine the concentration of a new macrolide derivative, ABT-229, in human plasma. The analyte was extracted using liquid-liquid extraction with a mixture of ethyl acetate and hexane at basic pH. Detection was highly specific for the analyte of interest, as evidenced by the lack of interference in the chromatograms of drug-free plasma extracts. The lower limit of quantitation was 0.10 ng/mL based on 1 mL of extracted plasma. The linear calibration range of the procedure extended from 0.10 to 199 ng/mL. The method proved reliable and rugged; within-run and day-to-day relative standard deviations were less than 10%. The stability of ABT-229 under frozen storage, room temperature, and freeze/thaw conditions was found to be excellent. Plasma concentrations determined by the method were highly comparable with data obtained by electrochemical detection. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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