Flow Cytometric Measurement of Cold Agglutinins for Diagnosing Mycoplasma pneumoniae Pneumonia.
| Autor: | Kwan Yong Seo, Dong Il Won, Min Hyun Cho |
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| Předmět: |
MYCOPLASMA pneumoniae infections
AGGLUTINATION tests CHI-squared test STATISTICAL correlation ENZYME-linked immunosorbent assay FLOW cytometry IMMUNOLOGICAL adjuvants RESEARCH methodology POLYMERASE chain reaction PROBABILITY theory RESEARCH funding T-test (Statistics) DATA analysis DATA analysis software DIAGNOSIS |
| Zdroj: | Laboratory Medicine; Apr2012, Vol. 43 Issue 3, p68-73, 6p, 4 Charts, 4 Graphs |
| Abstrakt: | Background: Mycoplasma pneumoniae is associated with community-acquired pneumonia in children and adults. Although conventional cold agglutinin testing (using the manual cold agglutinins [CA] assay) has not been regarded as a valuable tool for diagnosis of M. pneumoniae, we have developed a new and efficient assay, flow CA, for flow cytometric measurement of cold agglutinins. Methods: Flow CA measures cold agglutinins, which bind to group O red blood cells, using immunoglobulin M- (IgM) specific secondary antibodies after incubation of the O cells with serum samples at 4°C for 60 minutes. Once optimal parameters were established for this assay, its diagnostic efficiency was verified using serum samples from groups of individuals with M. pneumoniae (n = 27) and non-M. pneumoniae (n = 68) pneumonia; we then compared the resulting data with those generated by testing using manual CA or the M. pneumoniae IgM enzyme-linked immunosorbent assay (ELISA). Results: For diagnosis of M. pneumoniae pneumonia, flow CA testing showed significantly higher sensitivity than manual CA (78% and 46%, respectively, P = .01) and showed significantly higher specificity than M. pneumoniae IgM ELISA (94% and 66%, respectively, P < 0.001). The diagnostic efficiency of flow CA (90%) was also higher than that of the other two types of assay (82% and 71%, respectively).Conclusion: Flow CA is simpler, more rapid, more sensitive, and more quantitative than the manual CA assay; its results are also more specific than those of M. pneumoniae IgM ELISA. Therefore, flow CA can replace manual CA for use in diagnosis of M pneumoniae pneumonia. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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