| Autor: |
Ma, Michael, Wu, Shuenn-Jue, Howard, Maureen, Borkovec, Alexej |
| Zdroj: |
In Vitro; Sep1984, Vol. 20 Issue 9, p739-742, 4p |
| Abstrakt: |
We report here that the use of murine thymoma cell EL-4 conditioned medium enhances hybridoma yield in a low-antigen dose in vitro immunization protocol. This improved protocol allowed the production of a panel of monoclonal antibodies to Drosophila yolk proteins using less than 1 nanomole of antigen. We believe this refinement will be valuable for the application of hybridoma technology to biologically active materials that are hard to isolate and purify due to their low concentration in the biological fluids. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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