Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica.

Autor: Garg, Lokesh, Bhandari, Narinder, Rani, Vijay, Bhojwani, Sant
Zdroj: Plant Cell Reports; 1996, Vol. 15 Issue 11, p855-858, 4p
Abstrakt: Immature endosperm of Acacia nilotica formed a nodular callus on MS medium supplemented with 2,4-D, BAP and CH. In the third passage on this medium, in the dark, the callus differentiated somatic embryos. The embryos germinated on MS only after 15 d pre-treatment on modified MS medium in which major salts were replaced by those of major salts of B medium and supplemented with glutamine, CH and CW. Triploid nature of the somatic embryos was confirmed by Feulgen cytophotometry. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index