A genotyping strategy based on incorporation and cleavage of chemically modified nucleotides.

Autor: Wolfe, Jia Liu, Kawate, Tomohiko, Sarracino, David A., Zillmann, Martin, Olson, Jeffrey, Stanton Jr., Vincent P., Verdine, Gregory L.
Předmět:
Zdroj: Proceedings of the National Academy of Sciences of the United States of America; 8/20/2002, Vol. 99 Issue 17, p11073, 6p, 6 Diagrams, 2 Charts, 15 Graphs
Abstrakt: Describes a polymerase-based genotyping strategy called incorporation and complete chemical cleavage of chemically-modified nucleotides. Synthesis of modified nucleotide; Polymerase chain reaction incorporation; Matrix-assisted laser desorption ionization/time-of-flight mass spectrometry.
Databáze: Complementary Index