| Autor: |
Schuster, C., Rode, G., Rabes, H. |
| Zdroj: |
Journal of Cancer Research & Clinical Oncology; 1985, Vol. 110 Issue 2, p98-102, 5p |
| Abstrakt: |
O-Methylguanine DNA transferase activity was investigated in liver proteins obtained at various intervals after partial hepatectomy and/or after hydroxyurea-induced synchronization of the liver cell cycle. Liver proteins were incubated with H-methylated calf thymus DNA as previously described by Pegg et al. (1981). The loss of O-methylguanine was measured by radiochromatography of DNA hydrolysates. The extent of O-methylguanine repair differed during the cell cycle: the activity increased in late G, reached a maximum in early S phase and declined in late S phase and GM. These results indicate that hepatocytes are endowed with an increased DNA repair capacity for this promutagenic lesion during the period of highest transformation sensitivity in the cell cycle. Though increased, however, this repair potential does not, because of its exhaustibility, appear to be sufficient to prevent initiation of transformation after high doses of alkylating carcinogens. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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