| Autor: |
Wenman, Wanda, Meuser, Renate, Nyugen, Quoc, Kilani, Ruhangiz, El-Shewy, Khalid, Sherburne, Richard |
| Zdroj: |
Parasitology Research; 1993, Vol. 79 Issue 7, p587-592, 6p |
| Abstrakt: |
The trophozoites of Giardia lamblia possess several protein antigens, predominant among them a protein of ∼32,000 Da. In the present study, we used monospecific antibodies that recognize this protein to demonstrate its presence on a variety of G. lamblia isolates from human and animal sources. Immune electron microscopy was used to localize 32-kDa antigen on the trophozoite membrane and disk. Immunofluorescent assays employing monospecific antibodies confirmed the presence of 32-kDa antigen on the membrane and disk and its absence on flagella or nuclei. The N-terminal 17 amino acids of the 32-kDa antigen are identical to α-1-giardin, a protein component of microribbons on the ventral disk. These results suggest that the 32-kDa immunodominant trophozoite antigen is α-1-giardin. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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