| Autor: |
Jorgensen, W., Waldron, S., Grath, J., Roman, R., Vos, A., Williams, K. |
| Zdroj: |
Parasitology Research; 1992, Vol. 78 Issue 5, p423-426, 4p |
| Abstrakt: |
An Australian Babesia bigemina vaccine strain was maintained in suspension culture for 40 days. Parasite growth was compared using two tissue-culture flask sizes (25 and 75 cm), four gas mixes (2%, 2.5%, 3% and 3.5% O; 5% CO; and the balance N) and four packed blood cell (PCV) volumes (7%, 9%, 13% and 18%). The best continuous parasite yields were obtained from suspension cultures in 75-cm flasks at a PCV of 13% and gas mixtures of 2%-3% O, 5% CO and the balance N. Parasite yields per millilitre of culture medium were 3 times those obtained in microaerophilous stationary-phase cultures. The method has thus far been used for 6 months to produce the Australian requirements for hve B. bigemina vaccine. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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