Predominant periportal expression of the fructose 1,6-bisphosphatase gene in rat liver: dynamics during the daily feeding rhythm and starvation-refeeding cycle.

Autor: Eilers, F., Modaressi, S., Jungermann, K.
Zdroj: Histochemistry & Cell Biology; 1995, Vol. 103 Issue 4, p293-300, 8p
Abstrakt: Expression of the gene of the key gluconeogenic enzyme fructose 1,6-bisphosphatase (FBPase) was studied in rat liver during the daily feeding cycle and during refeeding after starvation. Total abundance of FBPase mRNA could be quantified by Northern blotting analysis with a digoxigenin-labelled 40-mer oligonucleotide probe. The zonal localization could not be demonstrated by in situ hybridization under several varied conditions with theP-end-labelled oligonucleotide probably due to insufficient sensitivity but was demonstrated with aS-labelled cRNA probe; the latter was synthesized from a polymerase chain reaction (PCR)-amplified 751 bp cDNA fragment inserted into a pBluescript. During a normal 12∶12 h day/night rhythm (darkness with feeding from 1900 to 0700 hours), the total amount of FBPase mRNA stayed almost the same throughout the whole day. After 60 h of starvation the FBPase mRNA level decreased from a maximum at 1800 hours by approximately one-third at the end of refeeding at 0700 hours. Both during the normal feeding rhythm, after 60 h of starvation and during refeeding, i.e. under all conditions, FBPase mRNA was predominantly distributed in the periportal zone. The results clearly show that the preferentially periportal distribution of the FBPase enzyme activity is controlled mainly at a pretranslational level. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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