Reference interval for glycated haemoglobin in a Montevideo population using a spectrophotometric assay, and comparison with a chromatographic method.

Autor: Servetto, C., López, E., Queiruga, G., Pintos, C., Raymondo, S., Mastropierro, M., Demiczylo, V.
Zdroj: Acta Diabetologica; 1992, Vol. 29 Issue 2, p107-109, 3p
Abstrakt: The reference interval for glycated haemoglobin in 174 apparently healthy individuals from Montevideo City (mean 94, SD 7 μmol fructose/10 mg haemoglobin) was determined using the thiobarbituric acid spectrophotometric method. In diabetic patients ( n=222) the mean was significantly higher than in the control group (mean 117, SD 19 μmol fructose/10 mg haemoglobin). The spectrophotometric method for the determination of glycated haemoglobin was compared to a microcolumn kit method (Boehringer, Mannheim, FRG). The correlation for glycated haemoglobins was acceptable ( r=0.85; n=44; P<0.001). The colorimetric reference interval for glycated haemoglobin was converted to an equivalent value in the chromatographic method, expressed as per cent HbA (mean 8.7%, SD 1.2%), using the least squares regression line ( y=0.603+ 0.0857 x). [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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