| Autor: |
Bidmon, H., Pitts, J., Solomon, H., Bondi, J., Stumpf, W. |
| Zdroj: |
Histochemistry; 1990, Vol. 95 Issue 1, p43-54, 12p |
| Abstrakt: |
Transdermal pathways and targets in the skin for estradiol were investigated using dry-mount autoradiography.H-estradiol-17β was applied at doses of 30.1 pmol, 120.4 pmol and 301 pmol/cm to shaved rat skin in the dorsal neck region. Vehicles were DMSO, ethylene glycol or sesame oil. After 2 h of topical treatment with 30.1 pmolH-estradiol×cm dissolved in DMSO a distinct cellular distribution was apparent. Target cells with concentrations of radioactivity were found in epidermis, sebaceous glands, dermal papillae of hair and fibroblasts. After treatment with 120.4 and 301 pmol/cm, a penetration gradient of radioactivity was recognizable however it masked specific cellular and subcellular uptake. The stratum corneum accumulated and retained radioactivity, apparently forming a depot for the hormone. Strong concentration and retention of the hormone was conspicious in sebaceous glands for more than 24 h, suggesting that sebaceous glands serve as a second storage site for the hormone. In all autoradiograms two penetration pathways to the dermis were visible: one through the stratum corneum and epidermis, the other through the hair canals and hair sheaths. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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