| Autor: |
Daimon, T., Mizuhira, V., Ono, M., Uchida, K. |
| Zdroj: |
Histochemistry; 1983, Vol. 77 Issue 3, p329-337, 9p |
| Abstrakt: |
A semiquantitative x-ray microanalytical study was made of dense bodies in rat and human blood platelets prepared by freeze substitution. After the freeze substitution, electron dense bodies containing 5-hydroxytryptamine(5-HT) were well preserved. Electron probe x-ray microanalyses clearly defined species differences in the cations of dense bodies of rat and man. Direct calculation of elemental concentration ratios demonstrated a high level of Mg, with lesser amounts of K and Ca in rat, while there was a negligible level of Mg in man but a high amount of Ca. These results indicate that freeze-substitution is a suitable preparation for the study of diffusible electrolytes which may open the way to quantitative analysis using appropriate standards for analytical electron microscopy of resin sections. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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