| Autor: |
Mcdonald, Hugh, weetall, Howard, Simon, Paula, Takeguchi, Milton, Odstrchel, Gerald |
| Zdroj: |
Journal of Solid Phase Biochemistry; 1978, Vol. 3 Issue 2, p151-160, 10p |
| Abstrakt: |
Antiserum specific for propanediol dehydrogenase, an enzyme found in Neisseria gonorrhoeae cells, has been immobilized to glass. When mixed with N gonorrhoeae cell lysates, the immobilized antibody (IMA) binds the enzyme. Over 70% of the calculated adsorbed activity can be recovered from the immobilized antibody-enzyme (IMA-E) complex. When mixed with bacterial lysates prepared from different organisms having propanediol dehydrogenase-like activity, the IMA specifically adsorbed the enzyme from the N gonorrhoeae lysate. IMA-E complexes have been prepared and their kinetic, temperature and chemical stability, and antigenic properties investigated. These studies demonstrated the feasibility of using an immobilized antibody in the detection of the propanediol dehydrogenase enzyme. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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