| Abstrakt: |
Using intraspecific hybrids, we have demonstrated the dominant nature of two phenotypic markers present in a mutant mouse 3T6 cell line, designated 3T6-V B2. These are, resistance to virus infection (V) and semiconstitutive synthesis of interferon (IF). Hybrids were formed by polyethylene glycol-mediated fusion between 3T6-V B2, or its parent 3T6, and 2TG0-13, a triply marked derivative of mouse 3T3 cells. When tested for the V marker, 3T6-V B2 × 2TG0-13 hybrid clones displayed a level of resistance to virus infection which was equal to or greater than that of 3T6-V B2. Similarly, when tested for the IF marker, these hybrid clones were found to possess the capacity to confer an interferon-induced antiviral state in mouse L cells upon cocultivation. By comparison, clones derived from 3T6 × 2TG0-13 fusions produced high levels of virus and failed to confer an interferon-induced antiviral state in L cells. [ABSTRACT FROM AUTHOR] |
